What media could you use to isolate Staphylococcus and why is this medium selective?

Posted by admin on September 2nd, 2009 and filed under media | 1 Comment »

What media could you use to isolate Staphylococcus and why is this medium selective?

MRS-medium
Mannitol Salt Agar

Salt (7,5%) will be the selective component in this medium. Staphylococcus is one of the few micro organisms who can tolerate such high levels of salt.

Mannitol will be the elective coponent, as only staphylococcus aureus can use it, and will cause a shift in the pH, turning an indicator from red to yellow

One Response

  1. Bram Fronik Says:

    MRS-medium
    Mannitol Salt Agar

    Salt (7,5%) will be the selective component in this medium. Staphylococcus is one of the few micro organisms who can tolerate such high levels of salt.

    Mannitol will be the elective coponent, as only staphylococcus aureus can use it, and will cause a shift in the pH, turning an indicator from red to yellow
    References :
    http://www.microbelibrary.org/Culture%20Media/details.asp?id=2315&Lang=

    http://biosci.usc.edu/courses/2002-fall/documents/bisc300-lab_media.pdf

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